Bio Rad Silver Stain Plus Protocol

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Silver staining is not recommended for agarose gels Completely destain the Coomassie stained gel in 40 methanol10 acetic acid Begin at step 2 of the silver staining protocol and proceed as usual Fix the gel in 30 methanol10 trichloroacetic acid35 sulfosalicylic acid for 1 hour.

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Silver staining is the most sensitive colorimetric method for detecting total protein.

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Please perform silver staining and Coomassie Brilliant Blue staining after elec- trophoresis.

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The heterochromatin under denaturing anionic dyes detectible under any or silver stain plus overlay will be performed

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In pathology silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections in temperature gradient gel electrophoresis and in polyacrylamide gels.

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